Some of the protocols used in the lab (mostly as PDF files)

• Cloning an expression construct
• Testing for expression and solubility
• Large scale expression with pBAT vectors
• Protein quantitation with UV
• SDS-PAGE
• Zinc-Imidazole staining for SDS-PAGE gels
• Inclusion body preparation
• Western blotting
• Hanahan competent cells
• RbCl competent cells

You might also find useful the following cloning, expression etc. related materials:

• pBAT series expression vectors
• Data sheet for an expression construct. Keep all the details of an expression clone in a single sheet of paper.
• Similar one for any clone/construct.
• Crystallisation record keeping form for 96-well plates
• Dima Chirgadze's 24-well format crystallisation record form
• Book-keeping forms for Hampton and Molecular Structures sparse matrix screens
• Various crystallisation screens in 96-well format

Slides and notes for the two talks I give annually in the postgraduate techniques lecture course:

• E.coli expression systems (PDF file)
• Fusion proteins and affinity purification (PDF file)